Sepsis real-time PCR 

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Sepsis PCR Testing screens for presence and detects microorganism nucleic acid products in a clinical sample offering the following advantages over conventional blood culture identification technologies:

  • Higher sensitivity and specificity
  • Improved identification of organisms that are difficult to culture 
  • Lower rates of false negatives in patients pre-treated with broad spectrum antimicrobials
  • Faster turnaround of results 

At ATCG Solutions we currently offer a 3-part pathogen diagnostics service using the Magicplex™ Sepsis Real-time PCR Technology:

Pathogen Screening:  with capacity to reveal the presence of up to 91 sepsis-causing pathogens in a clinical sample, namely”

  • 40 Streptococcus species
  • 3 Enterococcus species
  • 30 Staphylococcus species
  • 6 Gram-ve Bacteria-A species
  • 6 Gram-ve Bacteria-B species
  • 6 Fungus species

Pathogen Identification: with specific species identification of pathogens revealed in the screening phase of the same clinical sample.  Available species identification panels at this time will detect the following species:

Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia, Klebsiella pneumonia, Klebsiella oxytoca, Proteus mirabilis, Escherichia coli, Enterobacter cloacae, Enterobacter aerogenes, Candida albicans, Candida tropicalis, Candida parapsilosis.

The above are the 15 most prevalent sepsis-causing organisms in the Mulago National Referral Hospital setting in Uganda, as reported by Kateete et al; 2016 & Okoche et al; 2015

Molecular Characterization of Drug Resistant Pathogens: through PCR detection of known genetic markers of antibiotic drug resistance.

At the present time ATCG Solutions offers tests for the following 3 genetic markers expressed by Methicillin and Vancomycin resistant bacteria:

• VanA

• vanB  

• MecA 

Conditional upon additional research evidence and with evolving demands from clinician clients in the market, we aim to expand the range of pathogen species and of molecular markers identified by ATCG Solutions. At the moment we conduct testing from whole blood samples although we envisage processing from other clinical samples e.g CSF, urine, stool & pus. 

Our kits are CE marked for invitro diagnostics (IVD).


HIV 1- proviral DNA PCR &  HIV viral load 

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Quantitative PCR testing for HIV 1 proviral DNA detects complimentary HIV DNA that is present in blood cells soon after the establishment of HIV infection. 

HIV 1- proviral DNA PCR testing is beneficial in the following situations:

  1. Diagnosis of HIV Infection in infants below 2-years of age.
    • This is because standard antibody based “HIV tests” are not diagnostic in this age group.
  2. Diagnosis of early HIV-infection
    • HIV-DNA PCR reliably establishes HIV infection within 10-14 days of presumed HIV exposure, while standard antibody based HIV tests are only conclusive after a “window period” of up to 12 weeks from the time of presumed HIV infection. 
  3. Increasing efficiency
    • Rapid results enable quicker entry into care and treatment for high risk patients
  4. A 24 hr turn around time supports better clinical decisions and quick decisions can help reduce morbidity or mortility. 

Quantitative HIV-RNA PCR tests measure blood levels of HIV-RNA (also referred to as the HIV Viral Load)  in established HIV infection when the HIV virus is multiplying. 

Current Guidelines recommend HIV Viral Load Testing for evaluating & monitoring response and adherence to antiretroviral treatment for all HIV infected patients. 

WHO, CONSOLIDATED GUIDELINES ON THE USE OF ANTIRETROVIRAL DRUGS FOR TREATING AND PREVENTING HIV INFECTION. RECOMMENDATIONS FOR A PUBLIC HEALTH APPROACH. 2nd EDITION. JUNE 2016

UGANDA MINISTRY OF HEALTH, CONSOLIDATED GUIDELINES FOR PREVENTION AND TREATMENT OF HIV IN UGANDA. DECEMBER 2016

Our kits are CE marked for invitro diagnostics (IVD).

EQAS: Collage of American Pathologists


Hepatitis B (HBV) viral load 

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Quantitative HBV – DNA tests measure blood levels of HBV - DNA in established HBV infection when the HBV virus is actively multiplying. 

HBV viral load is increasingly used in the management of HBV infected patients to identify individuals with high viral replication in whom antiviral treatment is indicated & to monitor patients’ response to antiviral treatment.

Our kits are CE marked for invitro diagnostics (IVD).

EQAS: Collage of American Pathologists